α-凝血酶α-Thrombin選購指南

更新時間：2017-03-27

點擊次數：793

凝血酶Thrombin (Factor IIa)是一種絲氨酸蛋白酶，也是血液凝血級聯反應中的主要效應蛋白酶，顯現出促凝和抗凝的特性。凝血酶是由在凝血酶原復合物中的非活性凝血酶原在Xa因子（FXa）因子的作用下通過蛋白裂解的方式產生

【生理作用】當循環凝血因子在暴露的血管外組織與組織因子相接觸時，凝血酶會在組織上聚集。凝血酶通過激活血小板，催化纖維蛋白原轉化為纖維蛋白，促進血穩定而在血栓性疾病的引發和傳播上有著核心作用

α-凝血酶（α-Thrombin）是一種高度特異性的絲氨酸蛋白酶，由凝血酶原（prothrombin，II因子）經蛋白水解活化而來。它在凝血過程中起著非常重要的作用，不僅能夠促使纖維蛋白凝塊的產生，還負責提供反饋信號來激活前輔因子：V因子和VIII因子。也可以激活XIII因子和血小板，或者用作血管收縮劑。

在體內，活化的X因子（Factor Xa）切割凝血酶原，釋放出活性肽并將凝血酶切割成具有催化活性的α-凝血酶。α-凝血酶由一條輕鏈（A chain）（Mw ~6,000）和一條重鏈（B chain）（Mw ~31,000）通過二硫鍵連接而成。某些情況下，α-凝血酶會發生自溶生成β-凝血酶和γ-凝血酶。β-凝血酶由對α-凝血酶A鏈水解并切割含有B鏈糖基化位點的小片段（B1，B2）組合而成。

除了用于凝血研究之外，α-凝血酶還常用來位點特異性切割融合蛋白。基因重組時將凝血酶識別位點插入在目的蛋白與利于后續純化和/或表達的多肽或者蛋白之間，通過凝血酶切割表達的重組子即可釋放目的蛋白。凝血酶本身可通過親和層析技術快速去除。

作為生物活性物質的凝血酶：是一種在人體抗凝，促凝的凝血級聯反應中有著重要作用的生物活性蛋白。

產品性質

中文別名（Chinese synonym）	α-凝血酶；IIa 因子；
英文別名（English synonym）	α-Thrombin; Factor IIa;
CAS號（CAS NO.）	9002-04-4
分子量（Molecular weight）	37000 daltons
活力（Activity）	≥2700.00 NIH U/mg
緩沖液組分（Buffer）	50mM Sodium Citrate/0.2 M NaCl/0.1% PEG-8000/pH 6.5
消光系數（Extinction coefficient）（1%）	18.3

運輸和保存方法

粉末冰袋運輸，2-8℃保存。液體干冰運輸，≤-60℃保存。

凝血酶Thrombin (Factor IIa) 抗體

11571-05021	Thrombin, anti-human	150 ug	2800	AssayPro
11571-05011	Thrombin, anti-human	150 ug	2320	AssayPro
BP104C	Bovine anti-Thrombin (AT)	1 x 10 mg	4545	hyphen biomed
60130-05021	alpha-Thrombin (Factor IIa), anti-human	150 ug	2320	AssayPro
60130-05011	alpha-Thrombin (Factor IIa), anti-human	150 ug	2000	AssayPro
SAHT-AP	Anti-human Thrombin (F.IIa) sheep, AP IgG,	0.5 mg	5168	Enzyme Research2016
PAHT-S	Sheep anti-Human Thrombin	1x1 mg	436.8	Haematologic Technologies
PAHT-S	Sheep anti-Human Thrombin	1x5 mg	1506.96	Haematologic Technologies
SART-IG	Sheep anti rabbit Thrombin purified IgG	10 mg	2414	affinitybiologicals
SART-HRP	Sheep anti rabbit Thrombin peroxidase conjugated IgG	0.2 mg	4131	affinitybiologicals
SART-AP	Sheep anti rabbit Thrombin affinity purified IgG	0.5 mg	5304	affinitybiologicals
SAHT-IG	Sheep anti human Thrombin purified IgG	10 mg	2227	affinitybiologicals
SAHT-HRP	Sheep anti human Thrombin peroxidase conjugated IgG	0.2 mg	4029	affinitybiologicals
SAHT-APFTC	Sheep anti human Thrombin affinity purified, FITC conjugated IgG	0.1 mg	4029	affinitybiologicals
SAHT-APBIO	Sheep anti human Thrombin affinity purified, Biotinylated IgG	0.1 mg	4029	affinitybiologicals
SAHT-AP	Sheep anti human Thrombin affinity purified IgG	0.5 mg	5168	affinitybiologicals
SABT-IG	Sheep anti bovine Thrombin purified IgG	10 mg	2227	affinitybiologicals
SABT-HRP	Sheep anti bovine Thrombin peroxidase conjugated IgG	0.2 mg	4029	affinitybiologicals
SABT-AP	Sheep anti bovine Thrombin affinity purified IgG	0.5 mg	5168	affinitybiologicals
SART-IG	Anti-rabbit Thrombin (rF.IIa)sheep, purified IgG, 10 mg	10 mg	2600	Enzyme Research2016
SART-HRP	Anti-rabbit Thrombin (rF.IIa)sheep, perox-conj. IgG,	0.2 mg	4131	Enzyme Research2016
SART-AP	Anti-rabbit Thrombin (rF.IIa)sheep, affinity purified IgG,	0.5 mg	5304	Enzyme Research2016
SATAFI-AP	Anti-human Thrombin Activatable Fibrinolysis Inhibitor (TAFI)sheep, affinity purified IgG,	0.5 mg	5066	Enzyme Research2016
SAHT-HRP	Anti-human Thrombin (F.IIa)sheep, perox-conj. IgG,	0.2 mg	4029	Enzyme Research2016
SAHT-IG	Anti-human Thrombin (F.IIa) sheep, purified IgG,	10 mg	2600	Enzyme Research2016
SAHT-APFTC	Anti-human Thrombin (F.IIa) sheep, AP-FITC	0.1 mg	4029	Enzyme Research2016
AHT-5020	Anti-Human Thrombin	1x100 µg	1179.36	Haematologic Technologies
AHT-5020	Anti-Human Thrombin	5x100 µg	3057.6	Haematologic Technologies
SABT-IG	Anti-bovine Thrombin (bF.IIa)sheep, purified IgG,	10 mg	2600	Enzyme Research2016
SABT-HRP	Anti-bovine Thrombin (bF.IIa)sheep, perox-conj. IgG,	0.2 mg	4029	Enzyme Research2016
SABT-AP	Anti-bovine Thrombin (bF.IIa)sheep, affinity purified IgG,	0.5 mg	5168	Enzyme Research2016
SATAFI-IG	Anti-human Thrombin Activatable Fibrinolysis Inhibitor (TAFI)sheep, purified IgG,	10 mg	2635	Enzyme Research2016

凝血酶Thrombin (Factor IIa) 試劑盒

貨號	品名	包裝	價格	品牌
ET4010-1	Human Thrombin ELISA Kit	96 well plate	4760	AssayPro
Thrombin-HCII Complex	Thrombin-HCII Complex elisa kit	5 plate	8024	Enzyme Research2016
ADG844	OLIGOBIND® Thrombin activity assay	96 microwell plate	9200	American Diagnostica Inc

凝血酶Thrombin (Factor IIa) 抑制劑

貨號	品名	包裝	價格	品牌
TAFI-01	Thrombin-Activatable Fibrinolysis Inhibitor	1x50 µg	4298.83272	Haematologic Technologies
TAFI-01	Thrombin-Activatable Fibrinolysis Inhibitor	1x1 mg	68592.36384	Haematologic Technologies
TAFI-01	Thrombin-Activatable Fibrinolysis Inhibitor	5x50 µg	19250.2674	Haematologic Technologies
TAFI-01	Thrombin-Activatable Fibrinolysis Inhibitor	5x1 mg	308594.7774	Haematologic Technologies
TAFI-01	Thrombin-Activatable Fibrinolysis Inhibitor	10x1 mg	555305.2596	Haematologic Technologies
CK002L	HEMOCLOT Thrombin Inhibitors	3x2.5 mL	2130	hyphen biomed
CK002K	HEMOCLOT Thrombin Inhibitors	3x1 mL	1110	hyphen biomed

凝血酶Thrombin (Factor IIa) 發色底物

SN-17a	D-FPR-ANSNH-C6H11 • 2 HCl	100x1 mg	184235.688	Haematologic Technologies
SN-59	D-VPR-ANSNH-C4H9 • 2 HCl	1x1 mg	1842.35688	Haematologic Technologies
229005	BIOPHEN CS-01(81) - Thrombin Chromogenic Substrate	1 x 25 mg	3000	hyphen biomed
229001	BIOPHEN CS-01(38) - Thrombin Chromogenic substrate	1 x 25 mg	3000	hyphen biomed
0082032439	S-2238 H-D-Phe-Pip-Arg-pNA•2HCl	25mg	3500	Chromogenix2014
SN-20	Boc-L-FPR-ANSNH-C2H5	1x1 mg	1842.35688	Haematologic Technologies

凝血酶Thrombin (Factor IIa) ?其他耗材和試劑

ADG844T50	Thrombin blood collection tubes, 3 ml	50 tubes	5525	American Diagnostica Inc
ADG844T25	Thrombin blood collection tubes, 3 ml	25 tubes	3025	American Diagnostica Inc
CK011L	HEMOCLOT Thrombin Time (T.T.)	6 x 8 mL	915	hyphen biomed
CK011K	HEMOCLOT Thrombin Time (T.T.)	6 x 2 mL	285	hyphen biomed
CK001L	HEMOCLOT Thrombin (human)	6 x 8 mL	1155	hyphen biomed
CK001K	HEMOCLOT Thrombin (human)	6 x 2 mL	420	hyphen biomed

凝血酶Thrombin (Factor IIa) 蛋白

貨號	品名	包裝	價格	品牌
PT62082	Thrombin, Mouse	50 ug	3900	AssayPro
PP004K	Anti-Thrombin (AT)	6 x 150 µg	3030	hyphen biomed
PP004B	Anti-Thrombin (AT)	1 x 1.5 mg	1545	hyphen biomed
PP004A	Anti-Thrombin (AT)	1 x 150 µg	720	hyphen biomed
HT 1002a	Human a-Thrombin Priced per 1000 units	3,000+ units	2600	Enzyme Research2016
HT 1002a	Human a-Thrombin Priced per 1000 units	<3,000 units	2600	Enzyme Research2016
BT 1002a	Bovine Thrombin (Priced per 1000 units)	<3,000 units	2600	Enzyme Research2016
RIIa	Rat Thrombin	0.1 mg	5199.9192	Enzyme Research2016
RbIIa	Rabbit Thrombin	0.1 mg	5199.9192	Enzyme Research2016
PIIa	Porcine Thrombin	0.1 mg	5199.9192	Enzyme Research2016
MIIa	Murine Thrombin	0.05 mg	2600	Enzyme Research2016
MCT-5020	Mouse Thrombin	1x50 µg	4004.1456	Haematologic Technologies
MCT-5020	Mouse Thrombin	5x50 µg	17771.208	Haematologic Technologies
MCT-5020	Mouse Thrombin	1x1 mg	63116.34	Haematologic Technologies
MCT-5020	Mouse Thrombin	25x1 mg	1277277.456	Haematologic Technologies
MCT-5020	Mouse Thrombin	5x1 mg	283889.424	Haematologic Technologies
HTi	Human Thrombin Inactivated	1.0 mg	2600	Enzyme Research2016
EZ006O	Human Thrombin	1 x 10 NIH	195	hyphen biomed
EZ006K	Human Thrombin	6 x 10 NIH	825	hyphen biomed
EZ006B	Human Thrombin	1 x 1000 NIH	3765	hyphen biomed
HCGT-0021	Human gamma-thrombin	1x100 µg	992.03832	Haematologic Technologies
HCGT-0021	Human gamma-thrombin	5x100 µg	4487.7924	Haematologic Technologies
HCGT-0021	Human gamma-thrombin	5x1 mg	38736.7344	Haematologic Technologies
HCGT-0021	Human gamma-thrombin	10x1 mg	76292.4708	Haematologic Technologies
HCGT-0021	Human gamma-thrombin	50x1 mg	343670.418	Haematologic Technologies
HCGT-0021	Human gamma-thrombin	100x1 mg	670806.864	Haematologic Technologies
HCGT-0021	Human gamma-thrombin	500x1 mg	3035164.86	Haematologic Technologies
HCBT-0022	Human beta-thrombin	1x100 µg	992.03832	Haematologic Technologies
HCBT-0022	Human beta-thrombin	5x100 µg	4487.7924	Haematologic Technologies
HCBT-0022	Human beta-thrombin	5x1 mg	38736.7344	Haematologic Technologies
HCBT-0022	Human beta-thrombin	10x1 mg	76292.4708	Haematologic Technologies
HCBT-0022	Human beta-thrombin	50x1 mg	343670.418	Haematologic Technologies
HCBT-0022	Human beta-thrombin	100x1 mg	670806.864	Haematologic Technologies
HCBT-0022	Human beta-thrombin	500x1 mg	3035164.86	Haematologic Technologies
HCT-FPRCK	Human alpha-thrombin-FPRCK	1x100 µg	850.31856	Haematologic Technologies
HCT-FPRCK	Human alpha-thrombin-FPRCK	1x1mg	5172.77124	Haematologic Technologies
HCT-FPRCK	Human alpha-thrombin-FPRCK	5x100 µg	3542.994	Haematologic Technologies
HCT-FPRCK	Human alpha-thrombin-FPRCK	5x1mg	25509.5568	Haematologic Technologies
HCT-FPRCK	Human alpha-thrombin-FPRCK	10x1mg	46295.1216	Haematologic Technologies
HCT-FPRCK	Human alpha-thrombin-FPRCK	50x1mg	226751.616	Haematologic Technologies
HCT-FPRCK	Human alpha-thrombin-FPRCK	100x1mg	403901.316	Haematologic Technologies
HCT-DFP	Human alpha-thrombin-DFP	1x100 µg	850.31856	Haematologic Technologies
HCT-DFP	Human alpha-thrombin-DFP	1x1 mg	5172.77124	Haematologic Technologies
HCT-DFP	Human alpha-thrombin-DFP	5x100 µg	3542.994	Haematologic Technologies
HCT-DFP	Human alpha-thrombin-DFP	5x1 mg	25509.5568	Haematologic Technologies
HCT-DFP	Human alpha-thrombin-DFP	10x1 mg	46295.1216	Haematologic Technologies
HCT-DFP	Human alpha-thrombin-DFP	50x1 mg	226751.616	Haematologic Technologies
HCT-DFP	Human alpha-thrombin-DFP	100x1 mg	403901.316	Haematologic Technologies
HCT-BFPRCK	Human alpha-thrombin-BFPRCK	1x100 µg	850.31856	Haematologic Technologies
HCT-BFPRCK	Human alpha-thrombin-BFPRCK	1x1 mg	5172.77124	Haematologic Technologies
HCT-BFPRCK	Human alpha-thrombin-BFPRCK	5x100 µg	3542.994	Haematologic Technologies
HCT-BFPRCK	Human alpha-thrombin-BFPRCK	5x1 mg	25509.5568	Haematologic Technologies
HCT-BFPRCK	Human alpha-thrombin-BFPRCK	10x1 mg	46295.1216	Haematologic Technologies
HCT-BFPRCK	Human alpha-thrombin-BFPRCK	50x1 mg	226751.616	Haematologic Technologies
HCT-BFPRCK	Human alpha-thrombin-BFPRCK	100x1 mg	403901.316	Haematologic Technologies
HCT-0020	Human alpha-thrombin	1x100 µg	698.88	Haematologic Technologies
HCT-0020	Human alpha-thrombin	1x1 mg	2140.32	Haematologic Technologies
HCT-0020	Human alpha-thrombin	5x100 µg	2948.4	Haematologic Technologies
HCT-0020	Human alpha-thrombin	5x1 mg	9172.8	Haematologic Technologies
HCT-0020	Human alpha-thrombin	10x1 mg	16816.8	Haematologic Technologies
HCT-0020	Human alpha-thrombin	50x1 mg	81900	Haematologic Technologies
HCT-0020	Human alpha-thrombin	100x1 mg	146328	Haematologic Technologies
HCT-0020	Human alpha-thrombin	500x1 mg	677040	Haematologic Technologies
HGT	H. Gamma Thrombin	1.0 mg	6827.1048	Enzyme Research2016
CIIa	Canine Thrombin	0.1 mg	5199.9192	Enzyme Research2016
BT 1002a	Bovine Thrombin (Priced per 1000 units)	3,000+ units	2600	Enzyme Research2016
BE501K	Bovine Thrombin	10 x 500 NIH	14850	hyphen biomed
BE501C	Bovine Thrombin	1 x 500 NIH	1875	hyphen biomed
BE102K	Bovine Thrombin	6 x 21 NIH	885	hyphen biomed
BE102C	Bovine Thrombin	1 x 1000 NIH	3030	hyphen biomed
BE102B	Bovine Thrombin	1 x 100 NIH	735	hyphen biomed
BE102A	Bovine Thrombin	1 x 21 NIH	195	hyphen biomed
BCT-FPRCK	Bovine alpha-thrombin-FPRCK	1x200 µg	992.03832	Haematologic Technologies
BCT-FPRCK	Bovine alpha-thrombin-FPRCK	1x1 mg	3377.65428	Haematologic Technologies
BCT-FPRCK	Bovine alpha-thrombin-FPRCK	5x200 µg	4015.3932	Haematologic Technologies
BCT-FPRCK	Bovine alpha-thrombin-FPRCK	5x1 mg	16652.0718	Haematologic Technologies
BCT-FPRCK	Bovine alpha-thrombin-FPRCK	10x1 mg	29761.1496	Haematologic Technologies
BCT-FPRCK	Bovine alpha-thrombin-FPRCK	50x1 mg	145262.754	Haematologic Technologies
BCT-FPRCK	Bovine alpha-thrombin-FPRCK	100x1 mg	266905.548	Haematologic Technologies
BCT-DFP	Bovine alpha-thrombin-DFP	1x200 µg	992.03832	Haematologic Technologies
BCT-DFP	Bovine alpha-thrombin-DFP	1x1 mg	3377.65428	Haematologic Technologies
BCT-DFP	Bovine alpha-thrombin-DFP	5x200 µg	4015.3932	Haematologic Technologies
BCT-DFP	Bovine alpha-thrombin-DFP	5x1 mg	16652.0718	Haematologic Technologies
BCT-DFP	Bovine alpha-thrombin-DFP	10x1 mg	29761.1496	Haematologic Technologies
BCT-DFP	Bovine alpha-thrombin-DFP	50x1 mg	145262.754	Haematologic Technologies
BCT-DFP	Bovine alpha-thrombin-DFP	100x1 mg	266905.548	Haematologic Technologies
BCT-BFPRCK	Bovine alpha-thrombin-BFPRCK	1x200 µg	992.03832	Haematologic Technologies
BCT-BFPRCK	Bovine alpha-thrombin-BFPRCK	1x1 mg	3377.65428	Haematologic Technologies
BCT-BFPRCK	Bovine alpha-thrombin-BFPRCK	5x200 µg	4015.3932	Haematologic Technologies
BCT-BFPRCK	Bovine alpha-thrombin-BFPRCK	5x1 mg	16652.0718	Haematologic Technologies
BCT-BFPRCK	Bovine alpha-thrombin-BFPRCK	10x1 mg	29761.1496	Haematologic Technologies
BCT-BFPRCK	Bovine alpha-thrombin-BFPRCK	50x1 mg	145262.754	Haematologic Technologies
BCT-BFPRCK	Bovine alpha-thrombin-BFPRCK	100x1 mg	266905.548	Haematologic Technologies
BCT-1020	Bovine alpha-thrombin	1x200 µg	786.24	Haematologic Technologies
BCT-1020	Bovine alpha-thrombin	1x1 mg	2140.32	Haematologic Technologies
BCT-1020	Bovine alpha-thrombin	5x200 µg	3494.4	Haematologic Technologies
BCT-1020	Bovine alpha-thrombin	5x1 mg	9172.8	Haematologic Technologies
BCT-1020	Bovine alpha-thrombin	10x1 mg	16816.8	Haematologic Technologies
BCT-1020	Bovine alpha-thrombin	50x1 mg	81900	Haematologic Technologies
BCT-1020	Bovine alpha-thrombin	100x1 mg	146328	Haematologic Technologies
BCT-1020	Bovine alpha-thrombin	500x1 mg	677040	Haematologic Technologies
0081079639	AT III 1x25IU	1x25IU	5500	Chromogenix2014
0082072039	AT III 10x10 IU	10x10 IU	14780	Chromogenix2014
470HT	alpha-thrombin, human	2,500 unit	5628	American Diagnostica Inc

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